期刊
METABOLIC ENGINEERING
卷 51, 期 -, 页码 88-98出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymben.2018.10.004
关键词
C. cohnii; CM-ALE; Carbon relocation; Lipid; Cell growth
资金
- Tianjin Municipal Science and Technology Commission [15JCZDJC32500]
- National Natural Science Foundation of China [31770100, 21621004, 31370115, 91751102]
- National Basic Research Program of China (973. Program) [2014CB745101]
- Doctoral Program of Higher Education of China [20120032110020, 20130032120022]
- Zaoneng Biotechnology Inc.
Dietary omega-3 long-chain polyunsaturated fatty acids docosahexaenoic acid (DHA, C22:6) can be synthesized in microalgae Crypthecodinium cohnii; however, its productivity is still low. Here, we established a new protocol termed as chemical modulator based adaptive laboratory evolution (CM-ALE) to enhance lipid and DHA productivity in C. cohnii. First, ACCase inhibitor sethoxydim based CM-ALE was applied to redirect carbon equivalents from starch to lipid. Second, CM-ALE using growth modulator sesamol as selection pressure was conducted to relive negative effects of sesamol on lipid biosynthesis in C. cohnii, which allows enhancement of biomass productivity by 30% without decreasing lipid content when sesamol was added. After two-step CM-ALE, the lipid and DHA productivity in C. cohnii was respectively doubled to a level of 0.046 g/L/h and 0.025 g/L/h in culture with addition of 1mM sesamol, demonstrating that this two-step CM-ALE could be a valuable approach to maximize the properties of microalgae.
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