期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 51, 页码 35012-35020出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b12630
关键词
metal-affinity probe; immobilized metal ion affinity; metal oxide affinity; metal-organic framework; phosphorylated peptide
资金
- National Natural Science Foundation of China [21175134, 21375125, 21505134, 21675156]
- Creative Research Group Project of the National Natural Science Foundation of China [21321064]
- Instrument Developing Project of the Chinese Academy of Sciences [YZ201503]
- CAS Key Laboratory Foundation of Separation Science for Analytical Chemistry
The highly specific affinity between probes and phosphopeptides is the fundamental interaction for selective identification of phosphoproteomes that uncover the mechanisms of signal transduction, cell cycle, enzymatic regulation, and gene expression in biological systems. In this study, a metal-affinity probe possessing both interactions of metal oxide affinity chromatography (MOAC) and immobilized metal ion affinity chromatography (IMAC) was facilely prepared by immobilizing zirconium(W) on a zirconium organic framework of UiO-66-NH2, which holds dual metal centers of not only the inherent Zr-O cluster but also the immobilized Zr(IV) center. This dual-metal centered zirconium-organic framework (DZMOF) demonstrates as a highly specific metal-affinity probe toward the extraction of phosphopeptides due to the metal-affinity interactions of MOAC and IMAC toward either mono-phosphorylated or multi-phosphorylated peptides. The binding energies of zirconium 3d(5/2) and 3d(3/2) in this DZMOF are 183.07 and 185.47 eV, respectively, which are higher than those of the intact UiO-66-NH2 (182.84 and 185.17 eV, respectively), confirming the higher metal-affinity interaction between the DZMOF and phosphopeptides. This high metal-affinity probe presents an unprecedented strong performance in anti nonspecific interference during the capturing of phosphopeptides of beta-casein with the molar ratio of beta-casein vs bovine serum albumin up to ca. 1:5000. The enrichment of phosphopeptides from a human saliva sample by DZMOF further confirms the great potential of DZMOF in the extraction of low-abundance phosphopeptides for real complex biological samples.
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