期刊
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 94, 期 -, 页码 190-199出版社
ELSEVIER
DOI: 10.1016/j.msec.2018.09.021
关键词
Nanofibers; Electrospinning; Nerve guidance conduit (NGC); Polypyrrole (Ppy); Poly(l-lactic acid-co-epsilon-caprolactone)/silk fibroin
资金
- National Key Research Program of China [2016YFA0201702, 2016YFA0201700]
- National Natural Science Foundation of China [31470941, 31271035, 31370986]
- Shanghai Municipal Science and Technology Commission [15JC1490100, 15441905100]
- Yantai Double Hundred Talent Plan
- Opening Project of State Key Laboratory of Polymer Materials Engineering (Sichuan University) [sklpme2016-4-29]
- Fundamental Research Funds for the Central Universities [CUSF-DH-D-2017046, CUSF-DH-D-2017047]
According to our previous study, polypyrrole (Ppy) possessed good conductivity and non-cytotoxicity. In this study, the surface of electrospun poly(l-lactic-acid-co-epsilon-caprolactone)/silk fibroin (PLCL/SF) was coated with Ppy to fabricate Ppy-coated nerve guidance conduit (NGC). Firstly, the presence of Ppy on the prepared NGC was characterized and confirmed by scanning electron microscopy (SEM), X-ray photoelectron spectroscopic spectra (XPS) and Fourier transforms infrared spectroscopy (FTIR). Subsequently, Ppy-coated NGC was used to repair a 10 mm sciatic nerve gap in vivo. 4 and 12 weeks after implantation, the regenerated nerve tissues on defect sites were removed and sectioned for further evaluation. Histological analysis with hematoxylin-eosin (HE), toluidine blue (TB), and luxol fast blue (LFB) staining indicated that the coated Ppy could promote SCs proliferation in early post-surgery, and enhance myelin formation in later post-surgery. In consideration of immunofluorescence and morphology observation with SEM and TEM, it showed that the nerve regeneration of Ppy-coated NGC group was close to autograft group, which was better than PLCL/SF NGC. In addition, walking track analysis indicated that Ppy-coated NGC group showed a similar performance compared with the autograft group, and significantly better than PLCL/SF NGC group. These promising results showed the potential of Ppy-coated NGC in peripheral nerve regeneration.
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