期刊
MARINE DRUGS
卷 17, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/md17010052
关键词
sulfated galactofucan; fucoidan; anti-angiogenic activity; anti-tumor activity; Sargassum thunbergii
资金
- National Key Research and Development Program of China [2017YFE0103100]
- Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences [KF2018NO2]
- National Natural Science Foundation of China [41506165]
- Zhejiang Provincial Natural Science Foundation of China [LY19D060006]
Sulfated galactofucan (ST-2) was obtained from Sargassum thunbergii. It was then desulfated to obtain ST-2-DS, and autohydrolyzed and precipitated by ethanol to obtain the supernatant (ST-2-S) and precipitate (ST-2-C). ST-2-C was further fractionated by gel chromatography into two fractions, ST-2-H (high molecular weight) and ST-2-L (low molecular weight). Mass spectrometry (MS) of ST-2-DS was performed to elucidate the backbone of ST-2. It was shown that ST-2-DS contained a backbone of alternating galactopyranose residues (Gal)(n) (n 3) and fucopyranose residues (Fuc)(n). In addition, ST-2-S was also determined by MS to elucidate the branches of ST-2. It was suggested that sulfated fuco-oligomers might be the branches of ST-2. Compared to the NMR spectra of ST-2-H, the spectra of ST-2-L was more recognizable. It was shown that ST-2-L contain a backbone of (Gal)(n) and (Fuc)(n), sulfated mainly at C4 of Fuc, and interspersed with galactose (the linkages were likely to be 12 and 16). Therefore, ST-2 might contain a backbone of (Gal)(n) (n 3) and (Fuc)(n). The sulfation pattern was mainly at C4 of fucopyranose and partially at C4 of galactopyranose, and the branches were mainly sulfated fuco-oligomers. Finally, the anti-tumor and anti-angiogenic activities of ST-2 and its derivates were determined. It was shown that the low molecular-weight sulfated galactofucan, with higher fucose content, had better anti-angiogenic and anti-tumor activities.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据