期刊
MALARIA JOURNAL
卷 17, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12936-018-2501-4
关键词
Anopheles sinensis; AsinOrco; AsinOR10; 3-Methylindole
资金
- National Natural Science Foundation of China [81702034, 81471985, 81672059]
- China Postdoctoral Science Foundation [2017M622733]
- medical and health science and technology development project of Shandong Province [2016WS0393]
- Innovation Project of Shandong Academy of Medical Sciences
Background: Anopheles sinensis is an important vector for the spread of malaria in China. Olfactory-related behaviours, particularly oviposition site seeking, offer opportunities for disrupting the disease-transmission process. Results: This is the first report of the identification and characterization of AsinOrco and AsinOR10 in An. sinensis. AsinOrco and AsinOR10 share 97.49% and 90.37% amino acid sequence identity, respectively, with related sequences in Anopheles gambiae. A functional analysis demonstrated that AsinOrco-and AsinOR10-coexpressing HEK293 cells were highly sensitive to 3-methylindole, but showed no significant differences in response to other test odorants when compared to DMSO. Conclusions: AsinOrco was characterized as a new member of the Orco ortholog subfamily. AsinOR10, which appears to be a member of the OR2-10 subfamily, is directly involved in identification of oviposition sites. This finding will help to elucidate the molecular mechanisms underlying olfactory signaling in An. sinensis and provide many more molecular targets for eco-friendly pest control.
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