期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 14, 页码 8991-8997出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b01348
关键词
fluorescence imaging; cellular redox self-regulation; Ge-pyronines; reversible; fast-responsive; near-infrared
资金
- National Natural Science Foundation of China [21275018, 21575015, 21505004, 21203008]
- Beijing National Laboratory for Molecular Sciences (BNLMS) [20140121]
- Research Fund for the Doctoral Program of Higher Education of China (RFDP) [20121101110049]
- 111 Project [B07012]
Cellular self-regulation of reactive oxygen species (ROS) stress-via glutathione (GSH) antioxidant repair plays a crucial role in maintaining redox balance, which affects various physiological and pathological pathways. In this work, we developed a simple yet effective strategy for reversible, dynamic, and real-time fluorescence imaging of ROS stress and GSH repair, based on novel Ge-pyronine dyes (GePs). Unlike the current O-pyronine (OP) dye, the fluorescence of GePs can be quenched in GSH reduction and then greatly restored by ROS (e.g., CIO-, ONOO-, and HO center dot) oxidation because of their unique affinity toward thiols. The on-off' and off-on fluorescence switch can complete in 10 and 20 s, respectively, and exhibit excellent reversibility in vitro and in cells. GePs also show excitation in the long wavelength from the deep-red to near-infrared (NIR) (621-662 nm) region, high fluorescence quantum yield (Phi(fl) = 0.32-0.44) in aqueous media, and excellent cell permeability. Our results demonstrated that GePs can be used for real-time monitoring of the reversible and dynamic interconversion between ROS oxidation and GSH reduction in living cells. GePs might be a useful tool for investigating various redox-related physiological and pathological pathways.
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