期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 30, 页码 19371-19378出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b06671
关键词
bloodstream infection; bacteria; sepsis; aptamer; magnetic nanoparticle
资金
- National Natural Science Foundation of China [51272186, 21422105]
- A Foundation for the Author of National Excellent Doctoral Dissertation of P. R. China [201220]
- Ten Thousand Talents Program for Young Talents
Sepsis and bacteremia are life-threatening clinical syndromes associated with significant patient morbidity and mortality. Rapid and sensitive detection of pathogenic bacteria is the key to improve patient survival rates. Herein, we have rationally constructed a simple aptamer-based capture platform to shorten the time needed for confirmation of bacterial bloodstream infection in clinical blood samples. This capture platform is made of a mesoporons TiO2-coated magnetic nanoparticle and is modified with target aptamer. It features excellent bacterial enrichment efficiency of about 80% even at low bacterial concentrations (10-2000 CFU mL(-1)). More importantly, the bacteria can be enriched within 2 h, and the time for bacterial identification is effectively shortened in comparison to the gold standard in clinical diagnosis of bloodstream infection. The aptamer-based capture platform may pave a way for the detection of biomarkers and find potential applications in disease diagnosis.
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