期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 31, 页码 19892-19898出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b05406
关键词
fluorescent calcium-selective nanospheres; intracellular calcium; adjustable spectrum; continuous imaging; chromoionophore
资金
- 973 Program [2012 CB932600, 2013 CB933800]
- National Natural Science Foundation of China [21327902, 21135003, 21575060]
Continuous fluorescence imaging of intracellular ions in various spectral ranges is important for biological studies. In this paper, fluorescent calcium-selective nanospheres, including calix[4]arene-functionalized bodipy (CBDP) or 9-(diethylamino)-5-[(2-octyldecyl)-imino]benzo[a]phenoxazine (ETH 5350) as the chromoionophore, were prepared to demonstrate intracellular calcium imaging in visible or near-IR regions, respectively. The fluorescence of the nanospheres was controlled by the chromoionophore, and thus the spectral range for detection was adjustable by choosing the proper chromoionophore. The response time of the nanospheres to calcium was typically 1 s, which allowed accurate measurement of intracellular calcium. These nanospheres were loaded into cells through free endocytosis and exhibited fluorescence for 24 h, and their intensity was correlated with the elevation of intracellular calcium upon stimulation. The successful demonstration of calcium imaging by use of ion-selective nanospheres within two spectral ranges in 24 h supported that these nanospheres could be applied for continuous imaging of intracellular ions with adjustable spectra.
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