期刊
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
卷 1847, 期 6-7, 页码 587-598出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2015.03.007
关键词
Phospholipid; Mitochondrion; Mitochondrial DNA; Membrane plasticity
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/51906]
- Instituto Nacional de Ciencia e Tecnologia de Processos Redox em Biomedicina (INCT Redoxoma)
- Nucleo de Apoio a Pesquisa Redoxoma (NAP Redoxoma)
- Centro de Pesquisa, Inovacao e Difusao de Processos Redox em Biomedicina (CEPID Redoxoma) [13/07937-8]
- John Simon Guggenheim Memorial Foundation
- FAPESP [10/18254-0]
- FAPESP fellowships [13/04871-6]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [13/04871-6, 10/18254-0] Funding Source: FAPESP
Mitochondria play a key role in adaptation during stressing situations. Cardiolipin, the main anionic phospholipid in mitochondrial membranes, is expected to be a determinant in this adaptive mechanism since it modulates the activity of most membrane proteins. Here, we used Saccharomyces cerevisiae subjected to conditions that affect mitochondrial metabolism as a model to determine the possible role of cardiolipin in stress adaptation. Interestingly, we found that thermal stress promotes a 30% increase in the cardiolipin content and modifies the physical state of mitochondrial membranes. These changes have effects on mtDNA stability, adapting cells to thermal stress. Conversely, this effect is cardiolipin-dependent since a cardiolipin synthase-null mutant strain is unable to adapt to thermal stress as observed by a 60% increase of cells lacking mtDNA (rho(0)). Interestingly, we found that the loss of cardiolipin specifically affects the segregation of mtDNA to daughter cells, leading to a respiratory deficient phenotype after replication. We also provide evidence that mtDNA physically interacts with cardiolipin both in S. cerevisiae and in mammalian mitochondria. Overall, our results demonstrate that the mitochondrial lipid cardiolipin is a key determinant in the maintenance of mtDNA stability and segregation. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据