期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 9, 页码 5804-5812出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b11409
关键词
nanotoxicity; single cell; impedance spectroscopy; cell-communication; MEMS
资金
- National Natural Science Foundation [1334417]
- National Institute of Health of the U.S.A. [1R0IDA037838-01]
- Florida International University (FIU)
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [1334417] Funding Source: National Science Foundation
The traditional in vitro nanotoxicity assessment approaches are conducted on a monolayer of cell culture. However, to study a cell response without interference from the neighbor cells, a single cell study is necessary; especially in cases of neuronal, cancerous, and stem cells, wherein an individual cell's fate is often not explained by the whole cell population. Nonetheless, a single cell does not mimic the actual in vivo environment and lacks important information regarding cell communication with its microenvironment. Both a single cell and a cell population provide important and complementary information about cells' behaviors. In this research, we explored nanotoxicity assessment on a single cell and a small cell population using electrochemical impedance spectroscopy and a microelectromechanical system (MEMS) device. We demonstrated a controlled capture of PC12 cells in different-sized microwells (to capture a different number of cells) using a combined method of surface functionalization and dielectrophoresis. The present approach provides a rapid nanotoxicity response as compared to other conventional approaches. This is the first study, to our knowledge, which demonstrates a comparative response of a single cell and small cell colonies on the same MEMS platform, when exposed to metaloxide nanoparticles. We demonstrated that the microenvironment of a cell is also accountable for cells' behaviors and their responses to nanomaterials. The results of this experimental study open up a new hypothesis to be tested for identifying the role of cell communication in spreading toxicity in a cell population.
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