期刊
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
卷 51, 期 -, 页码 1-8出版社
ELSEVIER GMBH
DOI: 10.1016/j.jtemb.2018.09.001
关键词
IAPP; ProIAPP; Amylin; Amyloid: aggregation; Copper in biology; Aluminium in biology; Dynamic light scattering
资金
- Canadian Institute for Health Research
Islet amyloid polypeptide (IAPP(1-37)) or amylin is implicated in the aetiology of diabetes. It is found as amyloid along with its precursor ProIAPP(1-48) in the islets of Langerhans in the pancreas. Metals have been implicated in amyloidogenesis of both IAPP and ProIAPP. Herein we have used dynamic light scattering (DLS) to investigate how Al(III) and Cu(II) influence aggregation of ProIAPP(1-48) under near-physiological conditions and in a biologically-relevant timeframe. ProIAPP(1-48) formed primarily sub-micron particles within 5 min (e.g. 470 nm at 15 mu M peptide) that grew to micron-sized particles (1310 nm) within a 30 min timeframe. Equimolar Al(III) had little influence upon particle size at either 5 (656 nm) or 30 min (1250 nm) while Cu(II) tended to increase particle size over the same time period (731-1300 nm). It is suggested that any effects of Al(III) and Cu(II) reflected their well known tendencies to support beta-sheet or amorphous aggregates of ProIAPP(1-48) respectively.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据