期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 7, 页码 4477-4488出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.5b12660
关键词
binary colloidal crystals; human stem cells; osteogenesis; chondrogenesis; adipogenesis
资金
- Scientific Industrial Endowment Fund (SIEF)
- Australia Research Council (ARC) [PF12-026]
- Discovery Early Career Researcher Award (DECRA) [DE150101755]
- Australian Research Council [DE150101755] Funding Source: Australian Research Council
A new surface based on self-assembly of two colloids into well-defined nanostructures, so-called binary colloidal crystals (BCCs), was fabricated for stem cell culture. The facile fabrication process are able to cover large surface areas (>3 cm-diameter, i.e. > 7 cm(2)) with ordered surface nanotopographies that is often a challenge particularly in biomaterials science. From our library, four different combinations of BCCs were selected using mixtures of silica, polystyrene and poly(methyl methacrylate) particles with sizes in the range from 100 nm to 5 mu m. Cell spreading, proliferation, and surface-induced lineage commitment of human adipose-derived stem cells (hADSCs) was studied using quantitative real time polymerase chain reaction (qRT-PCR) and immunostaining. The results showed that BCCs induced osteo- and chondro- but not adipo-gene expression in the absence of induction medium suggesting that the osteochondral lineage can be stimulated by the BCCs. When applying induction media, higher osteo- and chondro-gene expression on BCCs was found compared with tissue culture polystyrene (TCPS) and flat silica (Si) controls, respectively. Colony forming of chondrogenic hADSCs was found on BCCs and TCPS but not Si controls, suggesting that the differentiation of stem cells is surface-dependent. BCCs provide access to complex nanotopographies and chemistries, which can find applications in cell culture and regenerative medicine.
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