期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 24, 页码 15113-15119出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b04112
关键词
magnetic hydrogel; cell-adhesive microarray interface; cell matrix interaction; cell cell interaction; multicellular spheroids; 3D cell culture
资金
- National Natural Science Foundation of China [61127002, 61420106012, 21273002]
- program for New Century Excellent Talents in University [NCET-09-0298]
- Suzhou medical apparatus and new medicine Fund [ZXY201440]
- research innovation plan of colleges and universities postgraduates of Jiangsu Province [CXLX12_0120]
Cell-adhesive properties are of great significance to materials serving as extracellular matrix mimics. Appropriate cell-adhesive property of material interface can balance the cell matrix interaction and cell cell interaction and can promote cells to form 3D structures. Herein, a novel magnetic polyacrylamide (PAM) hydrogel fabricated via combining magnetostatic field induced magnetic nanopatticles assembly and hydrogel gelation was applied as a multicellular spheroids culturing platform. When cultured on the cell-adhesive microarray interface of sliced magnetic hydrogel, normal and tumor cells from different cell lines could rapidly form multicellular spheroids spontaneously. Furthermore, cells which could only form loose cell aggregates in a classic 3D cell culture model (such as hanging drop system) were able to be promoted to form multicellular spheroids on this platform. In the light of its simplicity in fabricating as well as its effectiveness in promoting formation of multicellular spheroids which was considered as a prevailing tool in the study of the microenvironmental regulation of tumor cell physiology and therapeutic problems, this composite material holds promise in anticancer drugs or hyperthermia therapy evaluation in vitro in the future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据