期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 20, 页码 12702-12710出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b03422
关键词
lysosomes; two photon imaging iridium(III) complex; long-term tracking 3D tumor spheroid
资金
- 973 program [2015CB856301]
- National Science Foundation of China [21171177, 21471164, 21525105]
Lysosomes are the stomachs of the cells that degrade endocytosis and intracellular biomacromolecules and participate in various other cellular processes, such as apoptosis and cell migration. The ability of long-term tracking of lysosomes is very important to understand the details of lysosomal functions and to evaluate drug and gene delivery systems. For studying lysosomes, we designed and synthesized a water-soluble triscyclometalated iridium(III) complex (Ir-lyso) attaching morpholine moieties. The phosphorescent intensity of Ir-lyso is responsive to pH and decreases with an increase in the pH but not quenching in high pH. With excellent two-photon properties, Ir-lyso was used to light up the lysosomes in living cells and 3D tumor spheroids. Moreover, Ir-lyso could label lysosomes more than 4 days, so we developed this complex to act as a long-term probe for tracking lysosomes during cell migration and apoptosis. To the best of our knowledge, this is the first paradigm of metal complexes as the two -photon phosphorescent probe for long-term lysosomes tracking.
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