期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 47, 页码 32241-32249出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b11856
关键词
stem cells; tracking; nanoprobe; bone reparing; dual-energy CT
资金
- National Science Foundation of China [81301260, 31470958]
- Shanghai Jiao Tong University Medical Engineering Cross Grant [YG2013MS55]
- Shanghai Municipal Science and Technology Commission [13441902102]
- Global Innovation Initiative (GII) project - British Council
- Department of Business, Innovation and Skills in the United Kingdom
- Shanghai Pujiang Program [15PJD025]
- Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant [20152221]
- The British Council [GII207] Funding Source: researchfish
Transplant of bone marrow mesenchymal stem cells (BMSCs) has attracted considerable interest for bone regeneration. However, noninvasive and real-time tracking of location and concentration of the implanted BMSCs remains a big challenge. Herein we designed a novel approach involving the surface modification of gold nanoparticles (AuNPs) with silica layers and DNA Transfectin 3000 (TS) to improve biocompatibility and to enhance the uptake by BMSCs, hence rendering the ability of tracking BMSCs with dual-energy computer tomography (DECT). Results showed that the endocytosis of AuNPs@SiO2-TS by BMSCs was as high as similar to 255 pg/cell after one-day incubation and did not obviously decrease after 14 days. Meanwhile, the AuNPs@SiO2-TS had no influence on the viability, cell cycle, and capabilities on osteogenic, chondrogenic, and adipogenic differentiation of BMSCs. Under a bone-defect rabbit model, the DECT images showed the migration of BMSCs toward a cortical bone defect without variation in volume. This study demonstrated that AuNPs@SiO2-TS could be a potential cellular probe for noninvasive and real-time tracking of BMSCs in bone tissue repairs using clinical CT or DECT techniques. It provided a novel and intuitive methodology for observing and investigating the bone regeneration in clinic.
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