期刊
ACS APPLIED MATERIALS & INTERFACES
卷 8, 期 31, 页码 20432-20439出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsami.6b03284
关键词
hematite nanoparticle functionalization; protein immobilization; C-phycocyanin; laccase activity; immobilization enhances activity; surface functionalization
资金
- VELUX Foundation [790]
- SERI within COST action [TD1102]
Functional nanoparticles are valuable materials for energy production, bioelectronics, and diagnostic devices. The combination of biomolecules with nanosized material produces a new hybrid material with properties that can exceed the ones of the single components. Hematite is a widely available material that has found application in various sectors such as in sensing and solar energy production. We report a single-step immobilization process based on affinity and achieved by genetically engineering the protein of interest to carry a hematite-binding peptide. Fabricated hematite nanoparticles were then investigated for the immobilization of the two biomolecules C-phycocyanin (CPC) and laccase from Bacillus pumilus (LACC) under mild conditions. Genetic engineering of biomolecules with a hematite-affinity peptide led to a higher extent of protein immobilization and enhanced the catalytic activity of the enzyme.
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