Synthesis of Hollow Mesoporous Silica Nanorods with Controllable Aspect Ratios for Intracellular Triggered Drug Release in Cancer Cells

Here, we have reported a straightforward and effective synthetic strategy for synthesis of aspect-ratios controllable mesoporous silica nanorods with hollow structure (hMSR) and its application for transcription factor (TF)-responsive drug delivery intracellular. Templating by an acid degradable nickel hydrazine nanorods (NHNT), we have first synthesized the hollow dense silica nanorods and then coated on a mesoporous silica layer. Subsequently, the dense silica layer was removed by the surface-protected etching method and the hollow structure of hMSR was finally formed. The aspect ratios of the hMSR can be conveniently controlled by regulating the aspect ratios of NHNT. Four different hMSR with aspect ratios of ca. 2.5, ca. 5.3, ca. 8.1, and ca. 9.0 has been obtained. It was demonstrated that the as-prepared hMSRs have good stability, high drug loading capacity, and fast cell uptake capability, which makes them to a potential nanocarrier for drug delivery. As the paradigm, hMSR with an aspect ratio of ca. 8.1 was then applied for TF-responsive intracellular anticancer drug controlled release by using a Ag+-stabilized molecular switch of triplex DNA (TDNA) as capping agents and probes for TFs recognition. In the presence of TF, the pores of hMSR can be unlocked by the TFs induced disassembly of TDNA, leading to the leakage of DOX. The research in vitro displayed that this system has a TFs-triggered DOX release, and the cytotoxicity in L02 normal cells was lower than that of HeLa cells. We hope that this developed hMSR-based system will promote the development of cancer therapy in related fields.