期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 166, 期 -, 页码 281-290出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2019.01.020
关键词
Naozhenning granule; UHPLC-Qexactive quadrupole-orbitrap MS; Metabolites; Prototype components; Network pharmacology
资金
- Program of Science and Technology of Shanxi Province [201603D3113003, 2106ZD0404]
- Shanxi Provincial Education Department [2018JD05]
- Key Laboratory of Effective Substances Research and Utilization in TCM of Shanxi Province [201605D111004]
Naozhenning granule is a Chinese herbal formula, which is mainly used in the treatment of concussion, cerebral post-traumatic syndrome. Although its effectiveness has been certified in the clinical use, the mechanism of action and bioactive components of Naozhenning remain unknown. In this study, the ultrahigh performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry was applied to identify the absorbed constituents of Naozhenning in the rat serum. A total of 60 compounds, including 30 prototype components and 30 metabolites were identified. Then the absorbed constituents were subjected to the network pharmacology analysis. The compound-target-disease (CTD) and KEGG pathway analysis revealed that 40 absorbed constituents, 56 target genes and three key pathways such as RAS, PI3K/Akt, MAPK, TGF beta were probably related with the efficacy of Naozhenning against cerebral trauma and cerebral concussion. The results provided a scientific basis for understanding the bioactive compounds and the pharmacological mechanism of Naozhenning granule. (C) 2019 Elsevier B.V. All rights reserved.
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