Biodegradability and biodegradation pathways of chlorinated cyclodiene insecticides by soil fungi

An aerobic dieldrin-degrading fungus, Mucor racemosus strain DDF, and two aerobic endosulfan-degrading fungal strains, Mortierella sp. strains W8 and Cml-45, were isolated from soil contaminated with organochlorine pesticides. Strain DDF degraded more than 90% dieldrin during 10-days of incubation at 25 degrees and showed the production of a small amount of aldrin trans-diol. Moreover, strain DDF reduced levels of aldrin trans-diol while producing unknown metabolites that were determined to be aldrin trans-diol exo- and endo-phosphates. On the other hand, Mortierella sp. strains W8 and Cm1-45 degraded more than 70% and 50% of alpha and beta-endosulfan, respectively, over 28 days at 25 degrees C, in liquid cultures containing initial concentrations of 8.2 mu M of each substance. Only a small amount of endosulfan sulfate, a persistent metabolite, was detected in the both cultures, while these strains could not degrade endosulfan sulfate when this compound was provided as the initial substrate. Both strains generate endosulfan diol as a first step in the degradation of endosulfan, then undergo further conversion to endosulfan lac tone. (C) Pesticide Science Society of Japan