期刊
JOURNAL OF MASS SPECTROMETRY
卷 54, 期 1, 页码 66-72出版社
WILEY
DOI: 10.1002/jms.4305
关键词
chromones; covalent/noncovalent adduct; electrospray ionization; in-source reaction
资金
- National Natural Science Foundation of China [21527808, 21605002, 21775164]
- Beijing municipal high level innovative team building program [IDHT201805014]
Chromones were measured by using electrospray ionization mass spectrometry in negative mode. Interestingly, in addition to the deprotonated ion ([M - H](-)), unexpected [M + 17](-) and [M + 31](-) ions were observed in high intensity when water and methanol were used as the solvent. Chromones with different substitutes were tested. Compared with the deprotonated ion, [M + 17](-) and [M + 31](-) ions were observed with higher abundances when the C-3 site of chromones was substituted by electron withdrawing groups. Based on high performance liquid chromatography-mass spectrometry (LC-MS), deuterium-labeling and collisional-induced dissociation experiments, a covalent gas-phase nucleophilic addition reaction between chromone and water, and the formation of a noncovalent complex between chromone and methanol were proposed as the mechanism for the observed [M + 17](-) and [M + 31](-) ions, respectively. Understanding and using these unique gas phase reactions can avoid misannotation when analyzing chromones and their metabolites.
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