期刊
JOURNAL OF LIPID RESEARCH
卷 60, 期 2, 页码 412-420出版社
ELSEVIER
DOI: 10.1194/jlr.M090373
关键词
synthesis-secretion; Sprecher pathway; kinetics; fatty acid/metabolism; fatty acid/biosynthesis; omega-3 fatty acids; mass spectrometry
资金
- Natural Sciences and Engineering Research Council of Canada [482597]
- Ontario Graduate Scholarship
- Peterborough KM Hunter Charitable Foundation
- Fonds de Recherche du Quebec - Sante
- Bunge Ltd.
- Dairy Farmers of Canada
- Nestle Inc.
- SCIEX
- Arctic Nutrition
Tetracosahexaeoic acid (THA; 24:6n-3) is thought to be the immediate precursor of DHA in rodents; however, the relationship between THA and DHA metabolism has not been assessed in vivo. Here, we infused unesterified H-2(5)-THA and C-13(22)-DHA, at a steady state, into two groups of male Long-Evans rats and determined the synthesis-secretion kinetics, including daily synthesis-secretion rates of all 20-24 carbon n-3 PUFAs. We determined that the synthesis-secretion coefficient (a measure of the capacity to synthesize a given fatty acid) for the synthesis of DHA from plasma unesterified THA to be 134-fold higher than for THA from DHA. However, when considering the significantly higher endogenous plasma unesterified DHA pool, the daily synthesis-secretion rates were only 7-fold higher for DHA synthesis from THA (96.3 +/- 31.3 nmol/d) compared with that for THA synthesis from DHA (11.4 +/- 4.1 nmol/d). Furthermore, plasma unesterified THA was converted to DHA and secreted into the plasma at a 2.5-fold faster rate than remaining as THA itself (26.2 +/- 6.3 nmol/d), supporting THA's primary role as a precursor to DHA. In conclusion, using a 3 h infusion model in rats, we demonstrate for the first time in vivo that DHA is both a product and a precursor to THA.
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