期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 70, 期 1, 页码 343-356出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/ery360
关键词
Avirulence; biotrophy; effector-triggered susceptibility; pathogenicity; Phytophthora; RXLR effector; virulence
资金
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/G015244/1, BB/K018183/1, BB/L026880/1, BB/N009967/1]
- Scottish Government Rural and Environment Science and Analytical Services Division (RESAS)
- National Natural Science Foundation of China [31601600]
- China Postdoctoral Science Funding [2017M610214]
- Fundamental Research Funds for the Central Universities [2662017PY069]
- Postdoctoral Funding of Heilongjiang Province [LBH-Z16182]
- Fundamental Research Funds for the Central Universities of China [2662017PY069]
- China Scholarship Council (CSC)
- BBSRC [BB/S015663/1, BB/L008025/1, BB/L026880/1, BB/N009967/1, BB/G015244/1, BB/K018299/1, BB/K018183/1] Funding Source: UKRI
Oomycetes such as the potato blight pathogen Phytophthora infestans deliver RXLR effectors into plant cells to manipulate host processes and promote disease. Knowledge of where they localize inside host cells is important in understanding their function. Fifty-two P infestans RXLR effectors (PiRXLRs) up-regulated during early stages of infection were expressed as fluorescent protein (FP) fusions inside cells of the model host Nicotiana benthamiana. FP-PiRXLR fusions were predominantly nucleo-cytoplasmic, nuclear, or plasma membrane-associated. Some also localized to the endoplasmic reticulum, mitochondria, peroxisomes, or microtubules, suggesting diverse sites of subcellular activity. Seven of the 25 PiRXLRs examined during infection accumulated at sites of haustorium penetration, probably due to co-localization with host target processes; Pi16663 (Avr1), for example, localized to Sec5-associated mobile bodies which showed perihaustorial accumulation. Forty-five FP-RXLR fusions enhanced pathogen leaf colonization when expressed in Nicotiana benthamiana, revealing that their presence was beneficial to infection. Co-expression of PiRXLRs that target and suppress different immune pathways resulted in an additive enhancement of colonization, indicating the potential to study effector combinations using transient expression assays. We provide a broad platform of high confidence P infestans effector candidates from which to investigate the mechanisms, singly and in combination, by which this pathogen causes disease.
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