期刊
JOURNAL OF CHROMATOGRAPHIC SCIENCE
卷 57, 期 2, 页码 163-176出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/chromsci/bmy098
关键词
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资金
- Natural Science Foundation of China, China Postdoctoral Science Foundation [81560636, 81760702]
- JIANGXI Postdoctoral Science Foundation [2017M612159]
- Government of Jiangxi Province Health Department [2017KY07]
- Jiangxi Province R D projects [2016A142]
- [20161BBH80002]
Atractylodes macrocephala Koidz (AMK) is a traditional Chinese medicine widely used in the treatment of various diseases, especially spleen deficiency. As the principle active constituents of AMK, however, the metabolites of Atractylenolide-III (A-lactone-III) have not been identified in rats yet. In this study, a three-step high throughput method based on UHPLC-Q-TOF-MS-MS was developed to profile and characterize the metabolites of A-lactone-III in rat feces, urine and plasma. The initial step was a full-scan that utilized a multiple mass defect filter (MMDF) combined with dynamic background subtraction (DBS). PeakView((R))1.2 and Metabolitepilot1.5 software was then used to obtain data and seek possible metabolites. Finally, MS-MS spectra of the parent drug and possible metabolites were compared by the fragment ion peaks and retention times, which enabled metabolites to be identified. As a result, 53 metabolites were characterized in rats in vivo. The metabolic pathways of A-lactone-III were identified as including methylation, oxidation, hydroxylation, dihydroxylation, hydrogenation, glycosylation, sulfonation, and glucuronide, cysteine and N-acetylcysteine conjugation.
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