期刊
BIOCHEMISTRY
卷 54, 期 41, 页码 6299-6302出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.biochem.5b00954
关键词
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资金
- Campus Research Board, American Heart Association
- Office of the Provost, University of Illinois at Urbana-Champaign
Mammalian plasma membrane proteins make up the largest class of drug targets yet are difficult to study in a cell free system because of their intransigent nature. Herein, we perform direct encapsulation of plasma membrane proteins derived from mammalian cells into a functional nanodisc library. Peptide fingerprinting was used to analyze the proteome of the incorporated proteins in nanodiscs and to further demonstrate that the lipid composition of the nanodiscs directly affects the class of protein that is incorporated. Furthermore, the functionality of the incorporated membrane proteome was evaluated by measuring the activity of membrane proteins: Na+/K+-ATPase and receptor tyrosine kinases. This work is the first report of the successful establishment and characterization of a cell free functional library of mammalian membrane proteins into nanodiscs.
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