期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 120, 期 4, 页码 5268-5276出版社
WILEY
DOI: 10.1002/jcb.27801
关键词
apoptosis; Bcl-2; caspase 3; colorectal cancer (CRC)
资金
- Social development project Foundation of Ningbo [2014C50068]
- scientific and technological project Foundation of Ningbo [2014C50068]
- Huamei Foundation of Ningbo NO. 2 Hospital [2017HMK38, 2016HMKJ26, 2017HMK09]
- Medical and health science and technology Foundation of Zhejiang Province [2018KY699, 2017KY594, 2017KY593, 2018KY690]
- National Natural Science Foundation of China Youth Found [81602703]
- Natural Science Foundation of Ningbo [2017A610145, 2016A610135, 2017A610158, 2013A610224]
- Medical Foundation of Ningbo [2011B10]
- Municipal key disciplines of Ningbo Hospital [2013-88]
Long noncoding RNAs (lncRNAs) have important functions in tumor development and progression, including colorectal cancer (CRC), but their roles are not completely understood. In this study, the roles of the lncRNA transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 pseudogene 1 (TPTE2P1), previously implicated in gallbladder cancer cell migration and invasion, were evaluated in CRC. In particular, quantitative polymerase chain reaction was used to quantify TPTE2P1 levels in tumor tissues and cell lines. The association between TPTE2P1 and survival was analyzed using the online tool OncoLnc. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, colony formation assays, and flow cytometry were used to evaluate the effects of TPTE2P1 on viability, cell cycle progression, and apoptosis. Signaling pathway proteins were quantitated by Western blot analysis. Finally, the role of TPTE2P1 was analyzed in vivo using mouse models. TPTE2P1 levels were significantly higher in CRC tissues than in adjacent normal tissues. Higher expression was associated with a poor survival rate. The silencing of TPTE2P1 led to cell cycle arrest at the S phase and thereby inhibited cell viability. TPTE2P1 knockdown also caused cancer cell apoptosis via the activation of the apoptosis regulator (BCL2)/caspase 3 signaling cascade. In addition, the inhibition of TPTE2P1 had suppressive effects on tumors in vivo. TPTE2P1 is upregulated in CRC and plays essential roles in the regulation of cell viability in vitro and tumor formation in vivo.
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