Improvement in infected wound healing in type 1 diabetic rat by the synergistic effect of photobiomodulation therapy and conditioned medium

We investigated the effects of photobiomodulation therapy (PBMT) and conditioned medium (CM) of human bone marrow mesenchymal stem cells (hBM-MSC) individually and/or in combinationon the stereological parametersand the expression of basic fibroblast growth factor (bFGF), hypoxia-inducible factor (HIF-1), and stromal cell-derived factor-1 (SDF-1) in a wound model infected with methicillin-resistant Staphylococcus aureus (MRSA) in diabetic rats.CM was provided by culturing hBM-MSCs. Type 1 diabetes mellitus (T1DM) was induced in 72 rats, divided into four groups,harboring 18 rats each: group 1 served as a control group, group 2 received PBMT, group 3 received CM, and group 4 received CM+PBMT. On days 4, 7, and 15, six animals from each group were euthanized and the skin samples were separated for stereology examination and gene expression analysis by real-time polymerase chain reaction. In the CM+PBMT, CM, and PBMT groups, significant decreases were induced in the number of neutrophils (1460 +/- 93, 1854 +/- 138, 1719 +/- 248) and macrophages (539 +/- 69, 804 +/- 63, 912 +/- 41), and significant increases in the number of fibroblasts (1073 +/- 116, 836 +/- 75, 912 +/- 41) and angiogenesis (15230 +/- 516, 13318 +/- 1116, 14041 +/- 867), compared with those of the control group (2690 +/- 371, 1139 +/- 145, 566 +/- 90, 12585 +/- 1219). Interestingly, the findings of the stereological examination in the CM+PBMT group were statistically more significant than thosein the other groups. In the PBMT group, in most cases, the expression of bFGF, HIF-1, and SDF-1, on day 4 (27.7 +/- 0.14, 28.8 +/- 0.52, 27.5 +/- 0.54) and day 7 (26.8 +/- 1.4, 29.6 +/- 1.4, 28.3 +/- 1.2) were more significant than thosein the control (day 4, 19.3 +/- 0.42, 25.5 +/- 0.08, 22.6 +/- 0.04; day 7, 22.3 +/- 0.22, 28.3 +/- 0.59, 24.3 +/- 0.19) and other treatment groups. The application of PBMT + CM induced anti-inflammatory and angiogenic activities, and hastened wound healing process in a T1 DM model of MRSA infected wound.