期刊
JOURNAL OF CELLULAR BIOCHEMISTRY
卷 120, 期 6, 页码 9906-9916出版社
WILEY
DOI: 10.1002/jcb.28273
关键词
diabetes mellitus; diabetic foot ulcer; human bone marrow mesenchymal stem cell-conditioned medium; low-level laser therapy; methicillin-resistant staphylococcal aureus; phobiomodulation therapy; rat; real-time PCR; stereology
资金
- Zanjan Metabolic Diseases Research Center of Zanjan University of Medical Sciences [ZUMS.REC. A-12-977-1, A-12-977-2]
We investigated the effects of photobiomodulation therapy (PBMT) and conditioned medium (CM) of human bone marrow mesenchymal stem cells (hBM-MSC) individually and/or in combinationon the stereological parametersand the expression of basic fibroblast growth factor (bFGF), hypoxia-inducible factor (HIF-1), and stromal cell-derived factor-1 (SDF-1) in a wound model infected with methicillin-resistant Staphylococcus aureus (MRSA) in diabetic rats.CM was provided by culturing hBM-MSCs. Type 1 diabetes mellitus (T1DM) was induced in 72 rats, divided into four groups,harboring 18 rats each: group 1 served as a control group, group 2 received PBMT, group 3 received CM, and group 4 received CM+PBMT. On days 4, 7, and 15, six animals from each group were euthanized and the skin samples were separated for stereology examination and gene expression analysis by real-time polymerase chain reaction. In the CM+PBMT, CM, and PBMT groups, significant decreases were induced in the number of neutrophils (1460 +/- 93, 1854 +/- 138, 1719 +/- 248) and macrophages (539 +/- 69, 804 +/- 63, 912 +/- 41), and significant increases in the number of fibroblasts (1073 +/- 116, 836 +/- 75, 912 +/- 41) and angiogenesis (15230 +/- 516, 13318 +/- 1116, 14041 +/- 867), compared with those of the control group (2690 +/- 371, 1139 +/- 145, 566 +/- 90, 12585 +/- 1219). Interestingly, the findings of the stereological examination in the CM+PBMT group were statistically more significant than thosein the other groups. In the PBMT group, in most cases, the expression of bFGF, HIF-1, and SDF-1, on day 4 (27.7 +/- 0.14, 28.8 +/- 0.52, 27.5 +/- 0.54) and day 7 (26.8 +/- 1.4, 29.6 +/- 1.4, 28.3 +/- 1.2) were more significant than thosein the control (day 4, 19.3 +/- 0.42, 25.5 +/- 0.08, 22.6 +/- 0.04; day 7, 22.3 +/- 0.22, 28.3 +/- 0.59, 24.3 +/- 0.19) and other treatment groups. The application of PBMT + CM induced anti-inflammatory and angiogenic activities, and hastened wound healing process in a T1 DM model of MRSA infected wound.
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