期刊
JOURNAL OF CELL SCIENCE
卷 132, 期 2, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.214072
关键词
Dbf4; Gcn5; Cell cycle; Gene expression; Histone acetylation
类别
资金
- Purdue University Center for Cancer Research from the American Cancer Society [58-006-53]
- National Institutes of Health (NIH) [P30 CA023168]
- National Institute of General Medical Sciences [GM99945-01]
- NIH-funded Indiana Clinical and Translational Sciences Institute
- NATIONAL CANCER INSTITUTE [P30CA023168] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R35GM118068] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [P40OD018537] Funding Source: NIH RePORTER
Metazoans contain two homologs of the Gcn5-binding protein Ada2, Ada2a and Ada2b, which nucleate formation of the ATAC and SAGA complexes, respectively. In Drosophila melanogaster, there are two splice isoforms of Ada2b: Ada2b-PA and Ada2b-PB. Here, we show that only the Ada2b-PB isoform is in SAGA; in contrast, Ada2b-PA associates with Gcn5, Ada3, Sgf29 and Chiffon, forming the Chiffon histone acetyltransferase (CHAT) complex. Chiffon is the Drosophila ortholog of Dbf4, which binds and activates the cell cycle kinase Cdc7 to initiate DNA replication. In flies, Chiffon and Cdc7 are required in ovary follicle cells for gene amplification, a specialized form of DNA re-replication. Although chiffon was previously reported to be dispensable for viability, here, we find that Chiffon is required for both histone acetylation and viability in flies. Surprisingly, we show that chiffon is a dicistronic gene that encodes distinct Cdc7-and CHAT-binding polypeptides. Although the Cdc7-binding domain of Chiffon is not required for viability in flies, the CHAT-binding domain is essential for viability, but is not required for gene amplification, arguing against a role in DNA replication.
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