期刊
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
卷 145, 期 3, 页码 685-694出版社
SPRINGER
DOI: 10.1007/s00432-018-02827-6
关键词
Oral cancer; Saliva; Exosomes; Fourier-transform infrared (FTIR); Machine learning; Diagnosis
类别
资金
- Zvi Ferminger and wife Hana (Nee Cohen, zl) Fund for Cancer and Dental Research, Faculty of Medicine, Tel Aviv University
Purpose To determine the Fourier-transform infrared (FTIR) spectra of salivary exosomes from oral cancer (OC) patients and healthy individuals (HI) and to assess its diagnostic potential using computational-aided models. Methods Whole saliva samples were collected from 21 OC patients and 13 HI. Exosomes were pelleted using differential centrifugation (12,000g, 120,000g). The mid-infrared (IR) absorbance spectra (900-5000 cm(-1) range) were measured using MIR8025 Oriel Fourier-transform IR equipped with a PIKE MIRacle ZnSe attenuated total reflectance attachment. Machine learning techniques, utilized to build discrimination models for the absorbance data of OC and HI, included the principal component analysis-linear discriminant analysis (PCA-LDA) and support vector machine (SVM) classification. Sensitivity, specificity and the area under the receiver operating characteristic curve were calculated Results IR spectra of OC were consistently different from HI at 1072 cm(-1) (nucleic acids), 2924 cm(-1) and 2854 cm(-1) (membranous lipids), and 1543 cm(-1) (transmembrane proteins). The PCA-LDA discrimination model correctly classified the samples with a sensitivity of 100%, specificity of 89% and accuracy of 95%, and the SVM showed a training accuracy of 100% and a cross-validation accuracy of 89%. Conclusion We showed the specific IR spectral signature for OC salivary exosomes, which was accurately differentiated from HI exosomes based on detecting subtle changes in the conformations of proteins, lipids and nucleic acids using optimized artificial neural networks with small data sets. This non-invasive method should be further investigated for diagnosis of oral cancer at its very early stages or in oral lesions with potential for malignant transformation.
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