期刊
JOURNAL OF BIOPHOTONICS
卷 12, 期 4, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201800360
关键词
3-photon fluorescence imaging; 1700-nm window; osteocyte; third-harmonic generation imaging
资金
- Department of Education of Guangdong Province [2017KZDXM073]
- National Natural Science Foundation of China [61475103, 61775143]
- Science and Technology Innovation Commission of Shenzhen [KQJSCX20160226194151, JCYJ20160307150657874]
Osteocytes are the most abundant cells in bone and always the focus of bone research. They are embedded in the highly scattering mineralized bone matrix. Consequently, visualizing osteocytes deep in bone with subcellular resolution poses a major challenge for in vivo bone research. Here we overcome this challenge by demonstrating 3-photon imaging of osteocytes through the intact mouse skull in vivo. Through broadband transmittance characterization, we establish that the excitation at the 1700-nm window enables the highest optical transmittance through the skull. Using label-free third-harmonic generation (THG) imaging excited at this window, we visualize osteocytes through the whole 140-m mouse skull and 155 m into the brain in vivo. By developing selective labeling technique for the interstitial space, we visualize the sandwich structure of osteocytes in their native environment. Our work provides novel imaging methodology for bone research in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据