期刊
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
卷 14, 期 12, 页码 2156-2161出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2018.2655
关键词
Aptamer Electrochemical Biosensor; Mercury Ions; AuNPs; Detection
资金
- National Natural Science Foundation of China [61471168, 61571187, 61871180]
- China Postdoctoral Science Foundation [2016T90403, 2018M630498]
- Postdoctoral Science Foundation of Jiangsu Province [1601021A]
- Natural Science Foundation of Hunan Province [2017JJ2069]
- Hunan Key Research Project [2017SK2174]
A mercury ion aptamer electrochemical biosensor based on a Thymine-Hg2+-Thymine (T-Hg2+-T) structure has been constructed and successfully used to detect mercury ions in tap water samples. The aptamer electrochemical biosensor was assembled using a mercury ion aptamer-functionalized AuNPs-modified glass carbon electrode (aptamer/(AuNPs/CS)(2)/GCE) capable of specifically detecting mercury ions through a T-Hg2+-T structure. The experimental results indicated the optimum electrochemical performance of the prepared aptamer biosensor when the (AuNPs/CS)(2)/GCE surface was modified with 1.0 mu M aptamer and incubated with Hg2+ for 60 min. Moreover, the aptamer biosensor exhibits a good linear relationship between the logarithm of the Hg2+ concentration and the DPV peak current in the range from 0.01 to 500 nM following the linearization equation I-p (mu A) = 2.59902 +0.2097 logC (R-2 = 0.9994) with a limit of detection as low as 0.005 nM. Therefore, the constructed aptamer biosensor provides a simple and sensitive approach for Hg2+ detection in aqueous solution with promising application for trace Hg2+ detection in real samples.
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