期刊
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
卷 107, 期 6, 页码 1792-1805出版社
WILEY
DOI: 10.1002/jbm.b.34272
关键词
nerve regeneration; electrical stimulation; ionic conductivity; micro-nanofiber; scaffold
资金
- Connecticut Regenerative Medicine Research Fund [15-RMBUCHC-08]
- Division of Graduate Education [DGE-1747453]
- National Institute of Biomedical Imaging and Bioengineering [R01EB020640]
- National Science Foundation [EFRI-1332329]
- U.S. Department of Defense [OR120140]
Stem cell strategies and the use of electrical stimulation (ES) represent promising new frontiers for peripheral nerve regeneration. Composite matrices were fabricated by coating electrospun polycaprolactone/cellulose acetate micronanofibers with chitosan and ionically conductive (IC) polymers including, sulfonated polyaniline, and lignin sulfonate. These composite matrices were characterized for surface morphology, coating uniformity, ionic conductivity, and mechanical strength to explore as scaffold materials for nerve regeneration in conjunction with ES. Composite matrices measured conductivity in the range of 0.0049-0.0068 mS/m due to the uniform coating of sulfonated polymers on the micro-nanofibers. Thin films (2D) and composite fiber matrices (3D) of IC polymers seeded with human mesenchymal stem cells (hMSCs) were electrically stimulated at 0.5 V, 20 Hz for 1 h daily for 14 days to study the changes in cell viability, morphology, and expression of the neuronal-like phenotype. In vitro ES lead to changes in hMSCs' fibroblast morphology into elongated neurite-like structures with cell bodies for ES-treated and positive control growth factor-treated groups. Immunofluorescent staining revealed the presence of neuronal markers including beta 3-tubulin, microtubule-associated protein 2, and nestin in response to ES. (C) 2018 Wiley Periodicals, Inc.
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