Determination of biokinetic coefficients for nutrient removal from anaerobic liquid digestate by mixed microalgae

Anaerobic digestion is one of the most common methods used to generate energy from renewable sources. However, untreated anaerobic liquid digestate may cause eutrophication if directly discharged into the water bodies due to its high nutrient content. Microalgae can assimilate nutrients especially nitrogen and phosphorous from wastewater for their growth and produce valuable biomass. In this study, batch experiments were carried out to investigate the biokinetic coefficients for nutrient removal of mixed microalgae grown on anaerobic liquid digestate by Michaelis-Menten rate expression. The initial NH3-N concentration was varied between 18.6 and 87.1mgL(-1), while initial PO4-P concentration was between 1.85 and 6.88mgL(-1), which corresponds to 2%, 5%, 7%, and 10% dilution ratio of anaerobic digestate. According to the yield results (mg chl amg(-1) nutrient), mixed microalgae uptake 10 times more nitrogen than phosphorus. Biokinetic coefficients were determined as k(N)=2.48mg NH3-Nmg(-1) chl aday(-1), Km(N)=29.3mgL(-1), Y-N=0.45mg chl amg(-1) NH3-N for nitrogen; and k(P)=0.21mg PO4-Pmg(-1) chl aday(-1), Km(P)=2.94mgL(-1), YP=5.03mg chl amg(-1) PO4-P for phosphorus. The highest chlorophyll production (39mgL(-1) or 3.31mgL(-1)day(-1)) was observed at the highest dilution ratio of 10%. Moreover, the highest dilution resulted in highest biomass (1.25gL(-1)) despite of dark, high ammonicial, and particulate rich wastewater.