期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 126, 期 4, 页码 1290-1301出版社
WILEY
DOI: 10.1111/jam.14201
关键词
genetic recombination; genotype; norovirus; recycled water; sewage sludge
资金
- Thai Government Budget through Mahidol University, Bangkok, Thailand
Aims To investigate the presence of noroviruses in recycled water and sewage sludge obtained from a wastewater treatment plant in Bangkok Metropolitan Region, Thailand. Methods and Results Twenty-seven recycled water and twenty-three sewage sludge samples were tested for the presence of norovirus genogroup (G)I and GII using RT-nested PCR. Molecular characterization of noroviruses was undertaken by DNA sequencing and phylogenetic analysis. The level of the RNA genome of the noroviruses was determined using quantitative real-time RT-PCR. Noroviruses were detected in 44 center dot 4% of recycled water samples and 73 center dot 9% of sewage sludge samples. Norovirus GI.2 and GII.4 were identified in recycled water samples at levels of 2 center dot 19 x 10(1) and 3 center dot 26 x 10(4) RNA copies per litre. Six different genotypes of GI (GI.1, GI.2, GI.5a, GI.5b, GI.6b and GI.7) and GII.17 were identified in sewage sludge samples at levels ranging from 1 center dot 99 x 10(1)-1 center dot 43 x 10(5) RNA copies per gram wet weight. Four recombinant norovirus strains were detected in sewage sludge samples, namely GII.P16-GII.2, GII.P16-GII.4, GII.P16-GII.13 and GII.P21-GII.13. Conclusions These findings provide evidence that noroviruses may be spread to the community and environment via the use of recycled water for plant areas, and sewage sludge for land application. Significance and Impact of the Study This is the first study demonstrating recombinant norovirus strains in sewage sludge samples. The presence of noroviruses in recycled water and sewage sludge contributes to a health risk of environmental exposure.
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