期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 5, 页码 1381-1391出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b05408
关键词
Cara Cara orange juice; Bahia orange juice; gut microbiota; Clostridia; pyrosequencing; H-1 NMR-based metabolomics
资金
- Sao Paulo Research Foundation (FAPESP) [2016/08796-7]
- National Counsel of Technological and Scientific Development (CNPq) [150033/2015-0]
We have investigated the effect of intake of two different orange juices from Citrus sinensis cv. Cara Cara and cv. Bahia on faecal microbiota and metabolome using an integrated meta-omits approach. Following a randomized crossover design, healthy subjects daily consumed 500 mL of orange juice from Cara Cara or Bahia juices or an isocaloric control drink. Stools were collected at baseline (T0) and after a week (T7) of intervention. Operational taxonomic units (OTUs) were pyrosequenced targeting 16S rRNA, and faecal metabolites were analyzed by an untargeted metabolomics approach based on H-1 NMR spectroscopy. The major shift observed in microbiota composition after orange juice intake was the increased abundance of a network of Clostridia OTUs from Mogibacteriaceae, Tissierellaceae, Veillonellaceae, Odoribacteraceae, and Ruminococcaceae families, whose members were differently affected by Cara Cara or Bahia juice consumption. A core of six metabolites such as inositol, choline, lysine, arginine, urocanic acid, and formate significantly increased in Cara Cara compared to the Bahia group.
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