4.7 Article

Stable and Efficient Biosynthesis of 5-Aminolevulinic Acid Using Plasmid-Free Escherichia coli

期刊

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 5, 页码 1478-1483

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b06496

关键词

5-aminolevulinic acid; chemically induced chromosomal evolution; fermentation optimization; adaptative evolution; plasmid-free strain

资金

  1. National Natural Science Foundation of China [31670047, 31770095]
  2. Shandong Science and Technology Development Plan [2017GSF21121, 2017GSF21108]
  3. Natural Science Foundation of Shandong Province-Major Basic Research Project [ZR2017ZB0210]

向作者/读者索取更多资源

5-Aminolevulinic acid (5-ALA) is a key metabolic intermediate of the heme biosynthesis pathway, which has broad application prospects in agriculture and medicine. However, segregational instability of plasmid-based expression systems and low yield have hampered large-scale manufacture of 5-ALA. In this study, two important genes of the 5-ALA CS biosynthesis pathway, hemA and hemL, were integrated into Escherichia coli MG1655 for chemically induced chromosomal evolution (CIChE). The highest hemA and hemL copy-number, 98 per genome, was obtained in CIChE strain MG136. The 5-ALA titer of this strain reached 2724 mg/L in optimized condition. Then, after undergoing adaptative evolution and the deletion of recA, strain MG136a Delta recA::FRT could stably produce 4550 mg/L 5-ALA from glucose, 450 times the amount produced by hemA-hemL single copy strain MG1655-hemAL. This study constructed a plasmid-free E. coli strain for 5-ALA production, which will provide the basis for further manipulation of metabolic regulation and optimization of fermentation.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据