期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 67, 期 2, 页码 644-652出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b04810
关键词
peach; flavonol; MYB; FLS; transcriptional regulation; heterologous expression
资金
- National Natural Science Foundation of China [31872067]
- National Key R&D Program of China [2017YFE0102200]
- 111 project [B17039]
- Fundamental Research Funds for the Central Universities
Flavonoids are major polyphenol compounds in plants and contribute substantially to the health-promoting benefits of fruit and vegetables. Peach is rich in polyphenols with flavonols as the main flavonoids. To investigate the regulation of flavonol biosynthesis in peach fruit, two R2R3-MYB transcription factor (TF) genes, PpMYB1S and PpMYBF1, were isolated and characterized. Sequence analysis revealed that the PpMYB15 and PpMYBF1 proteins are members of the flavonol Glade of the R2R3-MYB family. Real-time quantitative PCR analysis showed that PpMYB1S and PpMYBF1 transcript levels correlated well with the flavonol content and the expression of flavonol synthase (PpFLS1) in different fruit samples. Dual-luciferase assays indicated that both PpMYB 1 5 and PpMYBF1 could trans-activate promoters of flavonoid biosynthesis genes, including chalcone synthase (PpCHS1), chalcone isomerase (PpCHI1), flavanone 3-hydroxylase (PpF3H), and PpFLS1. Transient overexpression of 35S::PpMYB15 or 35S::PpMYBF1 both triggered flavonol biosynthesis but not anthocyanin and proanthocyanidin biosynthesis in tobacco leaves. In transgenic tobacco flowers, overexpression of 35S::PpMYB15 or 35S::PpMYBF1 caused a significant increase in flavonol levels and significantly reduced anthocyanin accumulation, resulting in pale-pink or pure white flowers. These results suggest that PpMYB1S and PpMYBF1 are functional flavonol-specific positive regulators in peach fruit and are important candidates for biotechnological engineering flavonol biosynthesis in plants.
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