期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 66, 期 45, 页码 11926-11934出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.8b04330
关键词
adipogenesis; adipogenic transcription factors; hedgehog signaling; sulforaphene; 3T3-L1 adipocytes
资金
- National Research Foundation of Korea (NRF) [NRF-2017R1A2B4010685]
- Bio-Synergy Research Project of the Ministry of Science, ICT, and Future Planning [NRF2013M3A9C4078156]
Obesity is a risk factor for numerous metabolic disorders. In this study, we investigated the effects of the isothiocyanates sulforaphane (SA) and sulforaphene (SE) on adipogenesis in 3T3-L1 adipocytes. SE, a compound that is abundant in radish, inhibited adipogenesis by suppressing the adipogenic transcription factors peroxisome proliferator-activated receptor gamma (PPAR gamma, 69.2 +/- 2.4%, P < 0.05) and CCAAT/enhancer-binding protein alpha (C/EBP alpha, 36.1 +/- 3.1%, P < 0.05), thereby reducing fat accumulation in 3T3-L1 adipocytes (45.6 +/- 2.7%, P < 0.05); SA was less effective. SE exerted these activities through the activation of the Hedgehog (Hh) signaling pathway by restoring Smo ((2.1 +/- 0.2)-fold, P < 0.05) and Glil ((2.8 +/- 0.1)-fold, P < 0.05) expression, which was suppressed by adipogenic signals. These effects of SE were abrogated by treatment with the Hh inhibitor vismodegib. Thus, SE inhibits adipocyte differentiation via Hh signaling and may be an effective natural agent for preventing adipocyte hyperplasia and obesity.
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