期刊
ISME JOURNAL
卷 13, 期 5, 页码 1360-1369出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41396-018-0341-4
关键词
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资金
- Marine S& T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology [2018SDKJ0406-2]
- National Natural Science Foundation of China [31672279, 31722031, 91531306, 31572223]
- University of Chinese Academy of Sciences [Y8540XX1W2]
- Beijing Natural Science Foundation [JQ18020]
The single-celled ciliate Paramecium bursaria is an indispensable model for investigating endosymbiosis between protists and green-algal symbionts. To elucidate the mechanism of this type of endosymbiosis, we combined PacBio and Illumina sequencing to assemble a high-quality and near-complete macronuclear genome of P. bursaria. The genomic characteristics and phylogenetic analyses indicate that P. bursaria is the basal Glade of the Paramecium genus. Through comparative genomic analyses with its close relatives, we found that P. bursaria encodes more genes related to nitrogen metabolism and mineral absorption, but encodes fewer genes involved in oxygen binding and N-glycan biosynthesis. A comparison of the transcriptomic profiles between P. bursaria with and without endosymbiotic Chlorella showed differential expression of a wide range of metabolic genes. We selected 32 most differentially expressed genes to perform RNA interference experiment in P. bursaria, and found that P. bursaria can regulate the abundance of their symbionts through glutamine supply. This study provides novel insights into Paramecium evolution and will extend our knowledge of the molecular mechanism for the induction of endosymbiosis between P. bursaria and green algae.
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