期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 14, 期 -, 页码 1-15出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S176868
关键词
quaternary ammonium palmitoyl glycol chitosan; doxorubicin; artificial neural network; optical imaging; biodistribution; nanotheranostic
资金
- PAK-NORWAY Institutional Cooperation Program [PK3004]
- COMSTECH-TWAS project [12-198 RG/PHA/AS_C-UNESCO FR: 3240270874]
- Pakistan Science Foundation [PSF/Res/C-NILOP/Med (330)]
Purpose: This study was aimed to develop doxorubicin-loaded quaternary ammonium palmitoyl glycol chitosan (DOX-GCPQ) nanoformulation that could enable DOX delivery and noninvasive monitoring of drug accumulation and biodistribution at tumor site utilizing self-florescent property of doxorubicin. Materials and methods: DOX-GCPQ amphiphilic polymeric nanoformulations were prepared and optimized using artificial neural network (ANN) and characterized for surface morphology by atomic force microscopy, particle size with polydispersity index (PDI), and zeta potential by dynamic light scattering. Fourier transformed infrared (FTIR) and X-ray diffractometer studies were performed to examine drug polymer interaction. The ANN-optimized nanoformulation was investigated for in vitro release, cellular, tumor, and tissue uptake. Results: The optimized DOX-GCPQ nanoformulation was anionic spherical micelles with the hydrodynamic particle size of 97.8 +/- 1.5 nm, the PDI of < 0.3, the zeta potential of 28 +/- 2 mV, and the encapsulation efficiency of 80%+/- 1.5%. Nanoformulation demonstrated a sustained release pattern over 48 h, assuming Weibull model. Fluorescence microscopy revealed higher uptake of DOX-GCPQ in human rhabdomyosarcoma (RD) cells as compared to free DOX. In vitro cytotoxicity assay indicated a significant cytotoxicity of DOX-GCPQ against RD cells as compared to DOX and blank GCPQ (P < 0.05). DOX-GCPQ exhibited low IC50 (1.7 +/- 0.404 mu mol) when compared to that of DOX (3.0 +/- 0.968 mu mol). In skin tumor xenografts, optical imaging revealed significantly lower DOX-GCPQ in heart and liver (P < 0.05) and accumulated mainly in tumor (P < 0.05) as compared to other tissues. Conclusion: The features of nanoformulation, ie, small particle size, sustained drug release, and enhanced cellular uptake, potential to target tumor passively coupled with the possibility of monitoring of tumor localization by optical imaging may make DOX-GCPQ an efficient nanotheranostic system.
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