期刊
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
卷 16, 期 10, 页码 5725-5734出版社
SPRINGER
DOI: 10.1007/s13762-018-2155-3
关键词
Agro-industrial effluents; Bacillus subtilis RS1; Fermentation; Hexadecanoic acid; Polyhydroxyalkanoates
资金
- Chonbuk National University
The effectiveness of inexpensive agro-industrial wastes such as sugarcane molasses, paper mill effluent, and dairy effluent for the production of polyhydroxyalkanoates (PHAs) using Bacillus subtilis RS1 was assessed in this study. Laboratory-scale fermentations were carried out with both raw and pre-treated agro-industrial effluents, and maximum PHAs yield was observed in pre-treated effluents. Experimental variables such as pH, inoculum dose, and incubation time were further optimized to maximize PHAs yield, and the optimal conditions were identified as incubation time 48 h; pH 7; and inoculum dose 10% v/v. PHAs yield in pre-treated sugarcane molasses increased to 70.5% under optimal conditions. Fourier transform infrared spectroscopy and gas chromatography-mass spectrometric analysis revealed that the extracted polymer was composed of penta- and hexadecanoic acid methyl esters, a copolymer of PHAs. Results indicated that the pre-treated sugarcane molasses could be used as an inexpensive substrate for the production of PHAs.
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