期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 126, 期 -, 页码 309-315出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2018.10.029
关键词
Lytic polysaccharide monooxygenases; Aspergillus niger; Synergism; Hydrolytic boosting; Lignocellulose
资金
- National Natural Science Fundation of China [31770625]
- Key project of Natural Science Foundation of Tianjin [16JCZDJC31800]
Lytic polysaccharide monooxygenases (LPMOs) is a group of copper-dependent oxygenases that can cleave polysaccharides. Especially, the fungal LPMOs of Auxiliary Activity 9 (AA9) family can degrade cellulose to boost the lignocellulosic hydrolysis. LPMOs in Aspergillus niger, an important industrial producer of glycoside hydrolases, have not been studied. In this work, AnLPMO15g from A. niger was first cloned and expressed, and proved to be able to act on Avicel (R), straw, Carboxyl Methyl Cellulose (CMC) and xylan. MALDI-TOF/TOF mass spectrometry analysis indicated that it oxidatively cleaved the glycosidic bonds at Cl position. AnLPMO15g could obviously promote the catalytic efficiency of cellulase when acting on Avicel (R) and lignocellulose. The reducing sugar yield of Avicel (R) and straw catalyzed by AnLPMO15g together with cellulase were 1.93 and 2.31 times of those by cellulase only. The corresponding synergism degrees were 1.18 and 1.27 respectively. The increase in reducing sugar yield when AnLPMO15g acted together with xylanase on xylan was proved to be addition rather than synergism of these two enzymes. In conclusion, AnLPMO15g could be beneficial for plant cell wall decomposition and was a promising candidate to boost the efficiency of enzyme cocktails for lignocellulose degradation.
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