期刊
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
卷 41, 期 1, 页码 102-108出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08923973.2018.1555845
关键词
Inflammation; natural phenazines; Pseudomonas aeruginosa; macrophages; immune response
资金
- National Council for Scientific and Technological Development (CNPq) [14/2013]
- CAPES/PROCAD-2013 [2951/2014]
Context: Pyocyanin is a typical Pseudomonas aeruginosa virulence factor, a common Gram-negative rod responsible for a wide range of severe nosocomial infections. There is evidence indicating that pyocyanin has multiple biological activities, but little is known about anti-inflammatory properties.Objective: This study investigated pyocyanin effect on nitric oxide and cytokine production in lipopolysaccharide (LPS)-activated murine peritoneal macrophages.Materials and methods: Macrophages were incubated in the presence and absence of pyocyanin (1, 5, 10, 50, and 100 mu M) with and without LPS (1 mu g/mL). Nitric oxide production was determined by Griess reagent and tumor necrosis factor (TNF)- and interleukin (IL)-1 production was assessed by enzyme-linked immunosorbent assay. In addition, pyocyanin effects on zymosan A-induced peritonitis in mice were evaluated.Results: Pyocyanin (5 and 10 mu M) decreased nitric oxide, TNF-, and IL-1 production independent of macrophage death. On the other hand, in vivo, pyocyanin (5 mg/kg) was not able to affect leukocyte migration into the site of inflammation.Discussion and conclusion: Thus, our findings suggest that pyocyanin exerts anti-inflammatory effects on murine peritoneal macrophages, downregulating nitric oxide, TNF-, and IL-1 levels, which seems to be independent of cell migration. These effects may represent a mechanism of immune evasion; nevertheless more detailed studies should be performed to confirm this hypothesis.
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