期刊
GLOBAL CHANGE BIOLOGY BIOENERGY
卷 11, 期 1, 页码 249-259出版社
WILEY
DOI: 10.1111/gcbb.12590
关键词
biocatalyst; d-xylose; hemicellulose hydrolysate; l-arabinose; metabolic engineering; Pseudomonas putida KT2440
资金
- Ministry of Science, Research and the Arts of Baden-Wurttemberg [753310-5-86 A, 7533-10-5-86 B]
- EU project Horizon 2020 AD GUT [686271]
Lignocellulosic biomass is the most abundant bioresource on earth containing polymers mainly consisting of d-glucose, d-xylose, l-arabinose, and further sugars. In order to establish this alternative feedstock apart from applications in food, we engineered Pseudomonas putida KT2440 as microbial biocatalyst for the utilization of xylose and arabinose in addition to glucose as sole carbon sources. The d-xylose-metabolizing strain P. putida KT2440_xylAB and l-arabinose-metabolizing strain P. putida KT2440_araBAD were constructed by introducing respective operons from Escherichia coli. Surprisingly, we found out that both recombinant strains were able to grow on xylose as well as arabinose with high cell densities and growth rates comparable to glucose. In addition, the growth characteristics on various mixtures of glucose, xylose, and arabinose were investigated, which demonstrated the efficient co-utilization of hexose and pentose sugars. Finally, the possibility of using lignocellulose hydrolysate as substrate for the two recombinant strains was verified. The recombinant P. putida KT2440 strains presented here as flexible microbial biocatalysts to convert lignocellulosic sugars will undoubtedly contribute to the economic feasibility of the production of valuable compounds derived from renewable feedstock.
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