Effect of different homogenisation methods and UHT processing on the stability of pea protein emulsion

Pea protein is a very popular source of edible plant-based protein among legumes. In this study, the stability of ultra high temperature (UHT) processed pea protein emulsion prepared from 0.5 and 1.0% (w/v) pea protein concentrate (PPC) by two different homogenisation methods of microfluidisation (500 Bar) and ultrasonication (ultrasonicated for 1, 3 and 5 min) was investigated. In addition, the emulsion properties (particle and droplet size, flocculation, coalescence, zeta potential, hydrophobicity and creaming index) of PPC emulsions before and after UHT treatment were measured. The overall heat transfer coefficient (OHTC) versus time graphs were stable during UHT processing for both microfluidised and ultrasonicated PPC emulsions that indicates no fouling and good stability under the thermal treatment condition. Freshly prepared emulsion using 0.5 and 1.0% PPC and ultrasonicated for 5 min showed creaming index of 5.73 and 8.39%, particle size of 0.96 and 1.53 mu m respectively. In addition, the fat droplet size for the above samples measured 1.05 and 1.85 mu m for larger fat droplets and 0.51 and 0.72 mu m for smaller fat droplets, respectively. However, after UHT treatment this emulsion de-stabilised due to protein aggregation as indicated by the high flocculation index (13.22 and 103.35%), particle size (1.59 and 3.23 mu m) and droplet size (1.30 and 2.53 mu m, for large fat droplets and 0.90 and 1.22 mu m, for small fat droplets). After UHT treatment the microfluidised PPC emulsion using 0.5 and 1.0% PPC were the most stable with small particle size (2.85 and 0.36 mu m), high zeta potential (-56.36 and - 27.30) and low creaming index (3.87% and 4.97%), respectively as compared to ultrasonicated samples. Overall, this study revealed that UHT treatment improved emulsion properties of the microfluidised PPC emulsion compared to the ultrasonicated PPC emulsion.