期刊
FOOD CHEMISTRY
卷 270, 期 -, 页码 130-137出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2018.07.075
关键词
Deoxynivalenol; Monoclonal antibody; Enzyme-linked immunosorbent assay; Lateral-flow immunochromatographic assay; Cereal; Feed
资金
- National Key R D Program [2017YFC1601102]
- Natural Science Foundation of Jiangsu Province
- MOF [BE2016307, BK20150145, BK20140003, BE2014672, CMB21S1614, 201513006]
- Taishan Industry Leading Talent Special Funds
Ultrasensitive immunoassays, including an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral-flow immunochromatographic assay (ICA), were developed based on a monoclonal antibody for the analysis of deoxynivalenol in food and feed samples. With 0.01 M PBS, 20% ethanol-PBS, and 60% ethanol-PBS extraction, which are environmentally safe, the 50% inhibitory concentration (IC50) and limit of detection (LOD) values were 1.83-4.68 mu g/kg and 0.241-0.664 mu g/kg, respectively, with recovery rates of 87.7%-137% and coefficient variation values of 3.99-9.88% (intra-assay) and 4.17-9.81% (inter-assay) for the ic-ELISA relative to the results obtained by liquid chromatography-tandem mass spectrometry (LC-MS). For the ICA strip, the visual LODs were 10-150 mu g/kg, the cut-off values were 50-750 mu g/kg, and the calculated LODs were 1.97-46.8 mu g/kg, with different sample extraction solutions, and the recovery rates were 66.7%-127%. These methods are sensitive, simple and safe, providing an auxiliary analytical tool for screening the massive samples in markets.
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