期刊
FISH & SHELLFISH IMMUNOLOGY
卷 84, 期 -, 页码 894-905出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2018.10.084
关键词
Hydrogen peroxide; Oxidative stress; Inflammatory response; Hepatotoxicity; Oreochromis niloticus
资金
- Central Public-interest Scientific Institution Basal Research Fund, Freshwater Fisheries Research Center, CAFS [2017JBFZ01]
- National Natural Science Foundation of China [31702318]
- Jiangsu Provincial Natural Science Foundation of China [BK20170218]
Oxidative stress has been implicated in the pathogenesis of many liver diseases in fish, but the molecular mechanism is still obscure. Here, we used hydrogen peroxide (H2O2) as a reactive oxygen species (ROS) to induce liver injury and assess underlying molecular mechanism linking oxidative stress and liver injury in fish. Tilapia were injected with various concentrations of H2O2 (0, 40, 120, 200, 300 and 400 mM) for 72 h. The blood and liver were collected to assay biochemical parameters and genes expression after 24, 48 and 72 h of injection. The results showed that treatments with higher H2O2 levels (300 and/or 400 mM) significantly increased the levels of GPT, GOT, AKP and MDA, and apparently decreased the levels of TP, ALB, SOD, GSH, CAT, GST and T-AOC throughout of the 72 h. The gene expression data showed that treatments with 200, 300 and/or 400 H2O2 suppressed Nrf2/keapl pathway and its downstream genes including ho-1, nqol and gsta, activated inflammatory response via enhancing the mRNA levels of nf-kappa b, tnf-alpha, it-1 beta and it-8, and attenuating i1-10 mRNA level, and caused immunotoxicity through downregulating the genes expression of c3, hep, Ism and 1gm for 24, 48 and/or 72 h. Additionally, there was a mild or strong increase in levels of nrf2 and its subsequent antioxidant genes or enzymes such as ho-1, nqol, gst, CAT and SOD in treatments with lower concentrations of H2O2 (40 or 120 mM) for 24 and/or 48 h. Overall results suggested that H2O2 hepatotoxicity was mainly concerned with lipid peroxidation, impairment antioxidant defense systems, inflammatory response and immunotoxicity, and Nrf2/Keap1 and NF-kappa B signaling pathways played important roles in oxidative stress-induced liver injury in fish.
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