期刊
FISH & SHELLFISH IMMUNOLOGY
卷 84, 期 -, 页码 787-794出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2018.10.068
关键词
CXC chemokine; CXCL_F6; Chemotaxis; Large yellow croaker (Larimichthys crocea); Inflammatory response
资金
- National Natural Science Foundation of China [U1605211, 31772874, 31802337]
- China Agriculture Research System [CARS-47]
- Yantai Marine Economic Innovation and Development Demonstration City Industrial Chain Collaborative Innovation Project [YHCX-SW-L-201703]
Chemokines are a superfamily of structurally related chemotactic cytokines exerting significant roles in regulating cell migration and activation. Currently, five subgroups of fish specific CXC chemokines, named CXCL_F1-CXCL_F5, have been identified in teleost fish. However, understanding of the functions of these fish specific CXC chemokines is still limited. Here, a new member of fish specific CXC chemokines, LcCXCL_F6, was cloned from large yellow croaker Larimichthys crocea. Its open reading frame (ORF) is 369 nucleotides long, encoding a peptide of 122 amino acids (aa). The deduced LcCXCL_F6 protein contains a 19-aa signal peptide and a 103-aa mature polypeptide, which has four conserved cysteine residues (C-28, C-30, C-88, and C-72), as found in other known CXC chemokines. Phylogenetic analysis showed LcCXCL_F6 formed a separate Glade with sequences from other fish species, tentatively named CXCL_F6, distinct from the clades formed by fish OCCL_F1-5 and mammalian CXC chemokines. The LcCXCL_F6 transcripts were constitutively expressed in all examined tissues and significantly up-regulated in the spleen and head kidney tissues by poly (I:C) and Vibrio alginolyticus. Its transcripts were also detected in primary head kidney leukocytes (HKLs), peripheral blood leucocytes (PBLs), and large yellow croaker head kidney (LYCK) cell line, and significantly up-regulated by poly(I:C), lipopolysaccharide (LPS), and peptidoglycan (PGN) in HKLs. Recombinant LcCXCL_F6 protein (rLcCXCL_F6) could not only chemotactically attract monocytes/macrophages and lymphocytes from PBLs, but also enhance NO release and expression of proinflammatory cytokines (TNF-alpha, IL-1 beta, and CXCL8) in monocytes/macrophages. These results indicate that LcCXCL_F6 plays a role in mediating the inflammatory response.
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