期刊
FEBS LETTERS
卷 592, 期 24, 页码 4051-4065出版社
WILEY
DOI: 10.1002/1873-3468.13282
关键词
ATF3; microRNA; ossification; osteogenic differentiation; osteoporosis
资金
- National Natural Science Foundation of China [81471403, 81871124, 81670558, 81800542]
- Postgraduate Innovation Fund of '13th Five-Year comprehensive investment', Tianjin Medical University [YJSCX201702]
- Science and Technology Development Fund of Tianjin Education Commission for Higher Education [2017KJ221]
Through their multiple targets, microRNAs (miRNAs) are involved in numerous physiological and pathological processes. In this study, miR-342-3p was found to be deregulated with ossification of ligament or osteoporosis. We demonstrate that silencing miR-342-3p impairs osteoblast activity and matrix mineralization, while over expression of miR-342-3p promotes osteoblast differentiation significantly. Moreover, miR-342-3p directly targets activating transcription factor 3 (ATF3), which inhibits transcription of pro-osteogenic differentiation-associated genes. In addition, there exists a higher frequency of methylation at the CpG island of the Enah/Vasp-Like (EVL) locus in undifferentiated pre-osteoblasts; however, demethylation of the EVL CpG island induces over expression of miR-342-3p during osteogenic differentiation. This study suggests that miR-342-3p may serves as a potential marker for diagnosis and treatment of ossification of ligament and osteoporosis.
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