Unravelling the characteristics of a heteropolysaccharide-protein from an Haloarchaeal strain with flocculation effectiveness in heavy metals and dyes removal

The production, characterization and potential application in heavy metals and dyes removal of a novel heteropolysaccharide-protein named, gpHb, produced by an Haloarchaeal strain Halogeometricum borinquense strain A52 were investigated. The highest gpHb yield of 13.96 +/- 0.32 g/L was produced under optimized conditions by response surface methodology. We focused on the characteristics and flocculation performance of gpHb. An important attribute of protein with 16 protein types identified that occupied a total content of 50.2% in the gpHb. Additionally, carbohydrate that occupied 30.4% of the total bioflocculant content consisted of three monosaccharides. Fourier transform-infrared spectroscopy indicated the presence of carboxyl, hydroxyl, amine, amide, and sulphate groups. To further study flocculation activities, factors such as bioflocculant dosage, temperature, pH, salinity and cations addition were tested. In comparison to the chemical flocculant polyaluminium chloride, gpHb maintain high activity at large range of salinity and its flocculation activity was higher on both sides of pH 7. Addition of trivalent cation mainly Fe3+ enhances the flocculating rate indicating that the bioflocculant is negatively charged. Its practical applicability was established for heavy metals and dyes removal from saline aqueous solutions. The highest removal efficiency was observed with Cr3+ (91.4%) and Ni2+ (89.60%) and with basic blue 3 (83.8%) and basic red (78.6%). The excellent flocculation activity of gpHb under saline condition suggests its potential industrial utility for treatment of textile and tannery wastewaters. [GRAPHICS] .