期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 53, 期 4, 页码 2151-2160出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.8b06246
关键词
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资金
- Natural Science Foundation of Jiangsu Province [BK20170830, BK20180498]
- Fundamental Research Funds for the Central Universities [30917011305]
- Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse (Nanjing University of Science and Technology) [30918014102]
- program of Thousand Talents Plan
- program of Jiangsu Provincial Distinguished Professorship
The effects of 2-ethylhexyl diphenyl phosphate (EHDPP) on cytotoxicity and mRNA expression, as well as its metabolism, were investigated using a chicken embryonic hepatocyte (CEH) assay. After incubation for 36 h, the lethal concentration 50 (LC50) was 50 +/- 11 mu M, suggesting that EHDPP is one of a small cohort of highly toxic organophosphate esters (OPEs). By use of a ToxChip polymerase chain reaction (PCR) array, we report modulation of 6, 11, or 16/43 genes in CEH following exposure to 0.1, 1, or 10 mu M EHDPP, respectively. The altered genes were from all. nine biological pathways represented on the ToxChip including bile acids/cholesterol regulation, glucose metabolism, lipid homeostasis, and the thyroid hormone pathway. After incubation for 36 h, 92.5% of EHDPP was transformed, and one of its presumed metabolites, diphenyl phosphate (DPHP), only accounted for 12% of the original EHDPP concentration. Further screening by use of high-resolution mass spectrometry revealed a novel EHDPP metabolite, hydroxylated 2-ethylhexyl monophenyl phosphate (OH-EHMPP), which was also detected in a human blood pool. Additional EHDPP metabolites detected in the human blood pool included EHMPP and DPHP. Overall, this study provided novel information regarding the toxicity of EHDPP and identified a potential EHDPP metabolite, OH-EHMPP, in both avian species and humans.
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